Composite

Part:BBa_K2760034:Design

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-14)


Lactobachill Plan B (L. rhamnosus)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 38
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1982
    Illegal BamHI site found at 1817
    Illegal BamHI site found at 1886
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1157
    Illegal NgoMIV site found at 1823
    Illegal NgoMIV site found at 2132
    Illegal AgeI site found at 844
    Illegal AgeI site found at 2912
    Illegal AgeI site found at 3024
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 776
    Illegal SapI.rc site found at 1512


Design Notes

    • Designed for L. rhamnosus


Source

It contains the the spaCBA promoter (BBa_K2760005), a L. rhamnosus GG ribosome binding site (BBa_K2760006), then the codon optimized sequence for protein nsrR (BBa_K2760023) which is the PyeaR promoter repressor, two stop codons and then another ribosome binding site (the same as earlier). After this ribosome binding site, follows a modified and optimized iLOV sequence (BBa_K2760024 ), two stop codons and a loop terminator (Terminator 667). A PyeaR promoter follows (BBa_K216005), the same ribosome binding site, the signal peptide 1, SP1-NSP4 (BBa_K2760000), afterwards, the mutated gp80 (mutated IL-6 receptor) human soluble protein (BBa_K2760002) and a His-Tag (BBa_K2760012), then two stop codons, the RBS and finally an optimized M-cherry (BBa_K2760029), stop codon, loop terminator 908 (BBa_K2760008).

References